This position for a Research Technician is open in the Department of Ophthalmology at Duke University working with Dr. Eleonora Lad, a retinal specialist and clinician-scientist in the Duke Center for Macular Diseases. Age-related macular degeneration (AMD) is the leading cause of vision loss in people over 55. This research includes Veteran’s Hospital, NIH, foundation grant- and industry-funded research in dry AMD, with a specific focus on understanding the role of biomarkers of disease progression (imaging, functional and blood-based).
The hired technician will be involved in focused studies for flow cytometry and single-cell RNA sequencing (ssRNAseq) analysis of monocytes and macrophages from patients with AMD. This work will involve processing and preparation of cell and tissue samples. Prior applied research experience is preferred but not required. Specific experience with flow cytometry and ssRNAseq methods is not required. Knowledge of good laboratory and good clinical practices is desirable but not required, but the successful applicant must be willing to learn and apply these practices. The successful applicant will have a strong educational foundation in the biological sciences (especially cell and molecular biology) and will be motivated, hard-working, have excellent critical thinking skills, embrace and understand safety and compliance protocols, and work well with others, including collaborating with research scientists, clinical research coordinators, and supervising faculty investigators. In addition to the specific focus on ssRNAseq and flow cytometry, this technician position may assist other research scientists on other projects, learning and applying additional techniques such as cell/tissue culture, RNA isolation and RT-PCR, protein extraction, Western blot, and ELISA.
The hired technician will also have the opportunity to work on projects outside of wet lab. The technician will be involved with grading tests of retinal visual function, such as microperimetry, for clinical trials through the Duke Reading Center. In this role, the technician will grade exams for clinical trial participants before and after treatment at various time points to help monitor retinal changes over time.
Specific Job Responsibilities - Conduct experiments, compile and analyze data, draw tentative conclusions, and present results to the PI, Dr. Lad, on a weekly basis; maintain an organized detailed laboratory notebook; develop plans for research experiments; develop protocols and/or optimize existing protocols as necessary; review laboratory methods manuals, scientific journals, abstracts and other literature for information applicable to research experiments; prepare chemicals, reagents and solutions; sustain sufficient inventory of material, supplies and equipment for performance of duties; clean and maintain facilities; process human tissue samples for imaging and analyses.
A 2 year commitment is preferred, but not necessary.
Interested applicants should contact Eleonora (Nora) Lad, M.D., Ph.D. at with a copy of resume or curriculum vitae.